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Thank you! 😃

You connect the light source to the spectrometer via a fiber. If you are asking about a direct attach light source we used to offer in the FLAME-CHEM systems., we still offer direct attach light source, but it only works with the FLAME-T and some other legacy spectrometers. Please reach out to our Technical Sales to discuss further.

I'm guessing you're not a scientist because it took me about 60 seconds to find out how to change the font.

OceanView is deficient on multiple levels. I guess it may be liked by those who don't care about what they are measuring, as they happily contribute to the irreproducible research.

@@checkle1 I think you are arrogant and certainly not a scientist. The OceanView SW and the spectrophotometers are deficient and poorly performing tools that one should better avoid. That's my best advice based on my extensive experience.

@@cico452 lol calm down you white knight, you

Currently, we are not actively engaged with this technology. However, our technical team has provided an answer to your question. "These are colorimetric sensor films that use a spectrometer to correlate color-based absorbance to some pH level based on a prior calibration. At the moment these are not fully commercialized, such that there is no supporting software or documentation as these are developmental platforms for an OEM to integrate into their system. Performance specifications are largely based on the specific hardware used to interrogate the sensor films.

In Ocean Insight OceanView software, QuickView mode is the graph view that allows users to see raw detector counts without any adjustments, calibration or compensation. While QuickView mode is useful for setting integration time and adjusting light levels, it is not a truly accurate representation of the incoming light because it does not take into account instrument response function, fixed pattern noise and baseline offset. Every Ocean Insight spectrometer has a unique instrument response function, or IRF. The IRF characterizes how the specific spectrometer responds to light across its wavelength range. This response is far from uniform: a spectrometer will produce a different response (here defined as the number of QuickView mode counts produced for a fixed number of photons) at every pixel. The IRF is non-uniform because of the cumulative effects of optical inefficiencies in the light path. These include, but are not limited to the following wavelength dependent effects: attenuation of light in the fiber optic cable; absorbance of light by the mirrors; grating efficiency; and detector response. The IRF for each spectrometer is unique, and cannot really be measured. However, it is possible to compensate for the IRF. The two common corrections are relative irradiance and absolute irradiance calculations. Irradiance is the measurement of radiant flux per unit area hitting or passing through a surface. An absolute irradiance measurement results in a spectrum that is accurate in both shape and magnitude. The y-axis scale becomes scaled in power or flux units like μW/nm or μW/cm2/nm, making it easy to calculate other power or energy values. Measurements in absolute irradiance mode require a calibration using a source with known power output. A spectrum is measured with the sampling optic (fiber tip, CC-3 cosine corrector, etc.) connected to the calibration light source, and is then compared to the known output power of the calibration light source. The calibration process generates a file with energy response data for each pixel in the CCD, given in μJ/count. Factoring in the surface area of the sampling optic and the integration time allows irradiance measurements in μW/cm2 to be reported (power=energy/time). Calibration is only possible if the absolute power output of the calibration light source is known, so if the light source calibration data is not given in the units μW/cm2/nm, it may not be a light source capable of calibrating for absolute irradiance measurements. Radiometric calibration can be done by the user with a radiometric calibration source like the one shown in the video or Ocean Insight can calibrate the spectrometer in our ISO/IEC 17025:2017-certified calibration lab using a NIST-traceable light source for which irradiance as a function of distance is known. This video walks the user through the steps required to radiometrically calibrate a spectrometer using one of Ocean Insight’s radiometric calibration sources. www.oceaninsight.com/products/light-sources/calibration-sources/radiometric-calibrated/

Hi Yogesh, Q: Is it possible to use SR2 spectrometer to quantify 2-3 microliter DNA/RNA samples? - A: The SR2 excels at absorbance measurements including measurements to quantify DNA/RNA. Measurements with volumes of 2 - 3 microliters would require a quartz or other UV transmissive cuvette (z dimension - 15 mm) that works with these small volumes. Q: How much does the SR2 cost? - A: Please contact us for details. The best way is to submit a request via our online form. www.oceaninsight.com/contact-us/quote-request/. Prices may be different depending on the region. Q: Dealers in India? - A: We have a Sales/Technical Support team in India, and also an established distributor. Please contact our office at +91 80-67475336.

Is “counts” some kind of unit?

@@qww12111 counts it's a relative unit based in the conversion of the A/D Converter, it's just to not to use absolute units because the spectrometer is not calibrated (in intensity). Counts is extensive used in detectors devices

The new Ocean ST is a great option for solar irradiance measurements. Similar to the components shown in the video, these measurements would require an ST spectrometer, fiber, cosine corrector and OceanView software. Radiometric calibration of the system can be done by the Ocean Insight Calibration Lab or by the user with a radiometrically calibrated light source as shown in the video. Please reach out to salesinfo@oceaninsight.com to discuss your application and to receive a quote for a system to measure solar irradiance.

Você precisa da assinatura espectral de cada um dos micronutrientes, calibrar algumas amostras e depois você faz a predição em maior número de amostras.

bro have you figured out how ?

Thank you :)

Which one are you interested in general purpose or the temp controlled ones?

The spectrometer outputs via RS-232 and USB the data

Flame Spectrometers

Thank you so much for taking the time to watch this video and provide your very valuable input! The tips you provided regarding absolute irradiance calibration clearly come from strong expertise in this area. You read our minds as we are currently working to create additional content around the topics you mentioned. For your question about increasing the integration time after applying the boxcar, I have asked the same question myself. We recommend setting the integration time before applying boxcar smoothing to the spectral data. When the boxcar is applied, even though it appears that we now have less intensity at the ~656 nm D-alpha deuterium line in this case, the light intensity hitting the detector has not changed. The signal levels only appear lower because we have applied a boxcar value that averages pixels with much lower intensity on either side of the intense D-alpha line. Even in the case where the detector behaves well with some saturation (very detector dependent), this saturation would still show up in the data as extreme non-linear behavior making the data in the region where saturation occurs inaccurate and not meaningful. If it is determined through testing that some level of saturation can be tolerated, the integration time could be increased as you described to improve SNR but the data at the saturated wavelengths would still inaccurate and not meaningful.

@@oceanoptics Thanks for your detailed response. I have 2 followup thoughts: 1) when I have used this calibration lamp spectrum for absolute irradiance calibration, I believe only the part of the spectrum that is below 400nm is 'kept' after stitching the UV calibration together with the Vis calibration. In this case, how much would it really matter if the detector was saturated at (some) wavelengths that are above 400 nm? 2) I always figured that it was important to NOT use Boxcar and (maybe less-so) scans to average in the calibration process. Given it uses a specific calibration radiation source, shouldn't it inherently be VERY stable in terms of spectral intensity? Or are the averaging functions only used to moderate 'noise' on the acquisition side of the process (i.e., the light path and internal electronics) in this context?

Glad it helped

We do have Color Measurements Systems. Here: www.oceaninsight.com/fluxdata/

Hi, is a link to to software downloads and information: www.oceaninsight.com/support/software-downloads/

Your are welcome

Hi, here is a link: www.oceaninsight.com/products/measurement--technique-bundles/absorbance-bundles/

You can access all software downloads here: www.oceaninsight.com/support/software-downloads/

Have you been able to locate the Seabreeze driver and firmware? I am facing the same issue, and I couldn't find any information on their websites. :(